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转录因子XBP1S对人肝癌HepG2细胞增殖及凋亡的影响
         
Effects of transcription factor XBP1S on cell proliferation and apoptosis of hepatoma HepG2 cells

摘    要
目的: 探讨人剪接型X盒结合蛋白1(X-box binding protein1spliced,XBP1S)对肝癌HepG2细胞增殖和凋亡的影响。方法: 应用衣霉素(tunicamycin,Tm)和毒胡萝卜素(thapsigargin,Tg)建立HepG2细胞的内质网应激(endoplasmic reticulum stress,ERS)模型。将XBP1S真核表达载体pcDNA3.1(-)-XBP1S和靶向XBP1S的RNA干扰质粒pSUPER-XBP1S转染HepG2细胞,MTT法检测细胞的增殖能力,荧光显微镜下观察细胞的形态学变化,FCM法检测细胞凋亡率,Western印迹法检测caspase12的表达。结果: 转染pSUPER-XBP1S可有效抑制细胞增殖,而转染pcDNA3.1(-)-XBP1S可促进细胞增殖。荧光显微镜下可见Tm处理组细胞出现细胞凋亡的形态学改变,进一步下调XBP1S的表达可使这一改变增强。对照组、Tm组、Tm+pSUPER-XBP1S转染组和Tm+pcDNA3.1(-)-XBP1S转染组细胞的凋亡率分别为5.21%、41.51%、52.15%和35.87%,差异有统计学意义(P<0.05)。HepG2细胞中caspase12的表达,Tm组高于对照组,Tm+pSUPER-XBP1S转染组高于Tm组,Tm+pcDNA3.1(-)-XBP1S转染组低于Tm组。结论: XBP1S可以促进肝癌HepG2细胞增殖,抑制或促进XBP1S表达可调节ERS介导的细胞凋亡。
标    签 肝肿瘤   X盒结合蛋白1   内质网应激   细胞增殖   细胞凋亡   细胞   HepG   Liver neoplasms   X-box binding protein 1   Endoplasmic reticulum stress   Cell proliferation   Apoptosis   Cell,HepG 2  
 
Abstract
Objective: To study the effects of X-box binding protein 1 spliced(XBP1S) on cell proliferation and apoptosis of hepatoma HepG2 cells.Methods: The endoplasmic reticulum stress(ERS) model of HepG2 cells was constructed and induced by tunicamycin(Tm) and thapsigargin(Tg).XBP1S eukaryotic expression vector pcDNA3.1(-)-XBP1S and target XBP1S RNA interference plasmid pSUPERXBP1S were transfected into HepG2 cells.Cell proliferation was measured by MTT assay.The morphology changes of cells were observed under a fluorescence microscope.The apoptosis was detected by flow cytometry.The expression of caspase12 protein was determined by Western blotting.Results: The proliferation of HepG2 cells was inhibited after transfection with pSUPER-XBP1S,but that was improved after transfection with pcDNA3.1(-)-XBP1S.The apoptotic morphologic changes were obvious in Tmtreated group,and this effect was enhanced by down-regulation of XBP1S expression.The apoptotic rates of cells in the control,Tm-treated,Tm plus pSUPER-XBP1S-transfected and Tm plus pcDNA3.1(-)-XBP1S-transfected groups were 5.21%,41.51%,52.15% and 35.87%,respectively(P<0.05).The expression levels of caspase12 were gradually increased in the control,Tm plus pcDNA3.1(-)-XBP1Stransfected,Tm-treated and Tm plus pSUPER-XBP1S-transfected groups.Conclusion: XBP1S can improve the proliferation of HepG2 cells.Up-or down-regulation of XBP1S expression level maybe regulate ERSmediated apoptosis of HepG2 cells.

中图分类号 R735.7   DOI 10.3781/j.issn.1000-7431.2011.01.003

 
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所属栏目 基础研究

基金项目 国家自然科学基金资助项目(编号:31040019);教育部留学回国人员科研启动基金资助项目[编号:教外司留(2009)1590号]

收稿日期 2010/7/12

修改稿日期 2010/10/9

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引用该论文: LIN Jian-wei,LIU Ping,XIANG Ting-xiu,LI Xiang-zhu,ZHAO Wen-jun,GUO Feng-jin. Effects of transcription factor XBP1S on cell proliferation and apoptosis of hepatoma HepG2 cells[J]. Tumor, 2011, 31(1): 11~16
林建炜,刘平,向廷秀,李祥柱,赵文君,郭风劲. 转录因子XBP1S对人肝癌HepG2细胞增殖及凋亡的影响[J]. 肿瘤, 2011, 31(1): 11~16


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参考文献
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