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EB病毒潜伏膜蛋白1介导的上皮-间质转化增强鼻咽癌的转移潜能
         
Epstein-Barr virus-latent membrane protein 1-mediated epithelial-mesenchymal transition enhances the metastatic potential of nasopharyngeal carcinoma

摘    要
目的:探讨EB病毒潜伏膜蛋白1(latent membrane protein 1,LMP1)和p38在鼻咽癌上皮-间质转化(epithelial -mesenchymal transition,EMT)中的调控作用,分析其介导的EMT与肿瘤转移的关系。方法:采用免疫组织/细胞化学法、RT-PCR和蛋白质印迹法等检测鼻咽癌组织及鼻咽癌细胞中E-cadherin、vimentin、LMP1和p38的表达,Transwell实验检测鼻咽癌细胞的侵袭和运动能力。结果:E-cadherin在鼻咽癌和鼻咽癌颈部转移淋巴结组织中的阳性表达率有降低趋势(P<0.01),而vimentin和LMP1的阳性表达率有升高趋势(P<0.001),p38的阳性表达率差异则无统计学意义(P>0.05)。鼻咽癌组织中LMP1与E-cadherin的表达呈负相关(P<0.05);鼻咽癌颈部转移淋巴结组织中LMP1与E-cadherin的表达无相关性(P>0.05),而与vimentin的表达呈正相关(P<0.05)。E-cadherin mRNA和蛋白在CNE1-GL和CNE2Z细胞中的表达减弱,而vimentin mRNA和蛋白的表达增强。LMP1蛋白在CNE1-GL和CNE2Z细胞中高表达,p38蛋白则在CNE1-GL中高表达。Transwell实验证明,LMP1能够促进鼻咽癌细胞的运动和侵袭能力(P<0.001)。结论:LMP1可诱导鼻咽癌EMT,增强鼻咽癌的转移潜能。
标    签 鼻咽肿瘤   EB病毒潜伏膜蛋白1   p38   上皮-间质转化   转移   Nasopharyngeal neoplasms   Epstein-Barr virus-latent membrane protein 1   p38   epithelial-mesenchymal transition   Metastasis  
 
Abstract
Objective:To investigate the effects of Epstein-Barr virus-latent membrane protein 1(LMP1) and p38 mediating epithelial-mesenchymal transition of nasopharyngeal carcinoma(NPC),and to elucidate its relationship with tumor metastasis.Methods:The expression levels of E-cadherin,vimentin,LMP1 and p38 in NPC tissues and cell lines were detected by immunohistochemistry,immunocytochemistry,RT-PCR and Western blotting.The invasion and migration abilities of NPC cell lines were examined by Transwell assay.Results:The positive expression rates of E-cadherin in different NPC tissues and lymph node metastases were decreased(P<0.01),whereas the positive expression rates of vimentin and LMP1 were increased(P<0.001).The difference in the positive expression rate of p38 was not statistically significant(P>0.05).The expression of LMP1 was negatively associated with the expression of E-cadherin in NPC tissues(P<0.05).In cervical lymph node metastasis,the expression of LMP1,which had no association with the expression of E-cadherin(P>0.05),was associated with the expression of vimentin(P<0.05).The expressions of E-cadherin mRNA and protein were suppressed in CNE1-GL and CNE2Z cells,whereas the expressions of vimentin mRNA and protein were increased.The expression level of LMP1 protein was high in CNE1-GL and CNE2Z cells,and the expression level of p38 protein was high in CNE1-GL cells.Transwell assay showed that LMP1 could promote the migration and invasion abilities of NPC cells(P<0.001).Conclusion:LMP1 can induce EMT and enhance the metastatic potential of nasopharyngeal carcinoma.

中图分类号 R739.63   DOI 10.3781/j.issn.1000-7431.2011.07.010

 
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所属栏目 临床研究

基金项目 广东省教育厅重点学科建设基金资助项目(编号:GX0305);湛江市科技计划项目(编号:2007C05017);广东医学院2007年度科研项目(编号:M2007008)

收稿日期 2010/10/25

修改稿日期 2011/1/17

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引用该论文: LI Rong,JING Min,LI Xiao-bing,ZHU Shao-ping,HUANG Pei-chun. Epstein-Barr virus-latent membrane protein 1-mediated epithelial-mesenchymal transition enhances the metastatic potential of nasopharyngeal carcinoma[J]. Tumor, 2011, 31(7): 627~632
李蓉,敬敏,黎小兵,朱少平,黄培春. EB病毒潜伏膜蛋白1介导的上皮-间质转化增强鼻咽癌的转移潜能[J]. 肿瘤, 2011, 31(7): 627~632


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