目的:探讨Ⅱ相代谢酶微粒体环氧化物水解酶EPHX1基因多态性和烟酒习惯及其相互作用与原发性肝癌易感性的关系.方法:采用病例-对照研究和聚合酶链式反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术对广西地区105例原发性肝癌患者及151例健康对照者的EPHX1第3外显子113密码子基因型进行检测,并调查研究对象的烟酒习惯.结果:EPHX1第3外显子113密码子Tyr/Tyr、Tyr/His、His/His3种基因型频率在病例组分别为27.62%、21.90%、50.48%,对照组则分别为21.19%、34.44%、44.37%,2组差异无统计学意义(P > 0.05);EPHX1第3外显子113密码子基因型为His/His的个体在吸烟因素存在时,发生肝癌的危险性增加,OR值为2.99(95%CI:1.29~6.91);在HBV阳性的人群中,吸烟和饮酒习惯与EPHX1易感基因型对肝癌的发生有明显的协同作用.结论:EPHX1第3外显子基因多态与吸烟、饮酒等环境因素的相互作用可能增加个体患肝癌的危险性.

Objective: This study intended to explore there lationships of the polymorphism of phase Ⅱ drug-metabo lizingen-zyme, microsomal epoxide hydrolase 1 (EPHX1) with the habits of smoking and alcohol drinking, and their in teractions on risk of developing prmiary hepato cellular carcinoma(HCC).Methods: The genotypes at codon 113 of exon 3 of gene EPHX1 were analyzed in 105 patients with HCC and 151 healthy controls in Guangxi Province by using polymerase chain reaction followed by restriction fragment length polymorphism(PCR-RFLP).The habits of smoking and alcohol drinking of all the subjects were investigated. Results: The frequencies of Tyr/Tyr, Tyr/H is and His/His genotypes at codon 113 of exon 3 of gene EPHX1 were 27.62%, 21.90%, and 50.48% for HCC patients and 21.19%, 34.44%, and 44.37% for healthy controls, respectively. There was no significant difference (P > 0.05).Smoking increased the risk of developing HCC for patients with His/His genotype. The ORvalue was 2.99 (95%CI:1.29-6.91).For the HBV-positive patients, polymorphism of EPHX 1 and the habits of smoking and alcohol drinking had synergistic effects on the development of HCC.Conclusion: The interaction between the environmental factors such as smoking and alcohol drinking and polymorphism of EPHX1 gene may increase the risk of developing HCC.