目的:研究S100A4基因差异表达与结直肠癌分化、转移及预后的关系。方法:应用免疫组织化学SP法检测87例结直肠癌组织及癌旁结直肠组织中S100A4蛋白,并分析S100A4蛋白表达与结直肠癌临床病理因素及术后5年生存率的关系;免疫细胞化学SP法检测3种不同分化程度的结直肠癌细胞Caco-2、HT29、HCT116的S100A4蛋白表达,RT-PCR检测该3种细胞的S100A4 mRNA表达水平。结果:在癌旁结直肠组织腺上皮中S100A4蛋白不表达;在结直肠癌组织中S100A4蛋白的表达率为64.4%(56/87);与癌旁结直肠组织比较,差异有统计学意义(P < 0.01)。S100A4蛋白在结直肠癌中的表达与临床分期、淋巴结转移和5年生存率有关(P < 0.05),与其他临床病理因素无关(P > 0.05);3种结直肠癌细胞均表达S100A4蛋白;S100A4 mRNA表达水平的顺序依次为:HCT116最高,HT29次之,Caco-2最低;三者S100A4 mRNA表达水平差异有统计学意义(P < 0.01)。结论:S100A4基因表达和结直肠癌的发生发展、分化、侵袭转移及预后密切相关;S100A4蛋白可作为判定结直肠癌临床病理特征及判断预后的重要指标之一,也可能作为新的生物标志物及治疗结直肠癌浸润转移的潜在靶目标。

Objective: To investigate the association of S100A4 gene expression with differentiation, metastasis and prognosis of colorectal carcinoma.Methods: The SP immunohistochemical method was used to detect the expression of S100A4 in 87 cases of colorectal carcinoma and 87 cases of adjacent colorectal tissues.The relationship between the expression of S100A4 and the clinicopathologic factors and post-operative five-year survival rate was analyzed.The expression of S100A4 in three colorectal carcinoma cell lines Caco-2, HT29, HCT116 with different differentiation degrees was detected by using the SP immunocytochemical method;the mRNA expressions of S100A4 was measured in the colorectal carcinoma cell lines by using RT-PCR.Results: There was no S100A4 gene expression in glandular epithelium of adjacent colorectal tissues.The positive expression rate of S100A4 was 64.4%(56/87) in colorectal carcinoma;there was significant difference in S100A4 gene expression between colorectal carcinoma and adjacent colorectal tissues(P < 0.01).The expression of S100A4 positively correlated with the clinical stages, lymph node metastasis and five-year survival rate(P < 0.05), but not with other clinicopathologic factors(P > 0.05).S100A4 protein was expressed in the three colorectal carcinoma cell lines.The expression of S100A4 mRNA was the highest in HCT116 cells, moderate in HT29 cells, and the lowest in Caco-2 cells.There were significant differences in the expression level of S100A4 mRNA of the three cell lines(P < 0.01).Conclusion: S100A4 gene was closely related with initiation and progression, differentiation, invasion, metastasis and prognosis of colorectal cancer.S100A4 might be an important predictor of the clinicopathologic features and prognosis of colorectal carcinoma;and might be expected to become a new biomarker and potential target for colorectal carcinoma treatment.