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溶血磷脂酸对人卵巢癌细胞生长和侵袭转移能力的影响
         
The effect of lysophosphatidic acid on growth, invasion and metastasis of ovarian cancer cells

摘    要
目的:探讨溶血磷脂酸(lysophosphatidic acid, LPA)对卵巢癌细胞系SKOV3、3AO、CAOV3的生长和侵袭转移能力的影响。方法:LPA干预卵巢癌细胞系SKOV3、3AO、CAOV3, 并观察其对细胞增殖生长的影响; Boyden小室体外侵袭实验检测细胞侵袭能力; ELISA 方法检测LPA 刺激卵巢癌细胞系CAOV3后细胞上清液中转移相关因子的表达情况。结果:LPA体外干预卵巢癌细胞系能明显促进细胞增殖生长, 且存在剂量依赖性。Boyden小室体外侵袭实验结果显示, 实验组3AO和CAOV3细胞的侵袭指数都高于各自的空白对照组, 不同浓度组的比较差异有统计学意义, 但2组细胞系之间的比较差异无统计学意义。LPA干预CAOV3后细胞上清液中细胞因子的含量随LPA 的浓度变化产生相应的变化。结论:LPA 体外干预卵巢癌细胞系能明显促进细胞的增殖生长, 且侵袭指数和转移相关因子的表达量均会产生相应的变化, 说明LPA 对卵巢癌的侵袭和转移可能具有重要的生物学意义。
标    签 卵巢肿瘤   肿瘤浸润   溶血磷脂酸   Ovarian neoplasms   Neoplasm invasiveness   Lysophosphatidic acid  
 
Abstract
Objective: To investigate the effect of lysophosphatidic acid (LPA) on growth, invasion and metastasis of ovarian cancer cell lines SKOV3, 3AO and CAOV 3. Methods: The ovarian cancer cell line 3AO, SKOV3 and CAOV3 were cultured and incubated with LPA at different concentrations for 2, 4, 6, 16 and 24h. The control group was incubated with PBS instead of LPA. The effect of LPA on cell proliferation was detected by MTT assay. The invasion ability of ovarian cancer cells was evaluated by the Boyden chamber invasion test in vitro. The secretion of cytokines in the supernatant of cultured CAOV3 was measured by ELISA after being stimulated by LPA.Results: LPA interference greatly enhanced cell growth in a concentration-dependent manner. Boyden chamber invasion test showed that the invasion indices of 3AO and CAOV3 test groups were all higher than those of corresponding control groups, respectively. The difference was significant between different concentration groups. But the difference was not significant between the 3AO and CAOV3 cell lines. The expression of cytokines in supernatant fluid of CAOV3 cells had corresponding changes after LPA interfe-rence at different concentrations. Conclusion: LPA interference significantly enhances the growth of ovarian cancer cells in vitro. The invasion indices and the levels of migration-related cytokines have corresponding changes after LPA interference at different concentrations. It indicates that LPA might have the biologic significance in invasion and metastasis of epithelial ovarian cancer.

中图分类号 R737.31   DOI 10.3781/.jissn.1000-7431.2008.09.007

 
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所属栏目 基础研究

基金项目 河北省科技厅基金资助项目(编号:07276101D-104)

收稿日期 2007/11/19

修改稿日期 2008/3/14

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备注陈文雪(1969-), 女(汉族), 博士, 副教授, 硕士生导师

引用该论文: CHEN Wen-xue,WU Xiao-hua,LIU Jiang,WANG Hui,WANG Ping,SHI Li,YANG Hui-chai. The effect of lysophosphatidic acid on growth, invasion and metastasis of ovarian cancer cells[J]. Tumor, 2008, 28(9): 746~750
陈文雪,吴小华,刘江,王辉,汪萍,史丽,杨会钗. 溶血磷脂酸对人卵巢癌细胞生长和侵袭转移能力的影响[J]. 肿瘤, 2008, 28(9): 746~750


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