目的: 探讨胃癌组织中白介素-12(IL-12)与白介素-18(IL-18)的表达,及其与胃癌临床病理特征、血管生成及预后的关系。方法: 选取50例胃癌标本,通过免疫组化检测IL-12与IL-18在胃癌组织中的表达,显微图像分析系统测定微血管密度。结果: IL-12与IL-18在胃癌中的阳性表达率分别为44%(22/50)和26%(13/50),IL-12与病理分化(P=0.029)、浸润深度(P=0.002)、淋巴结转移(P=0.002)、远处转移(P=0.003)和TNM分期(P=0.005)相关,IL-18与远处转移密切相关(P=0.001);多因素生存分析显示IL-12、MVD和T分期是胃癌的独立预后因素。结论: IL-12和IL-18阳性表达与肿瘤进展和转移有关,IL-12是提示胃癌患者预后不良的独立因素。

Objective: To examine the expressions of interleukin-12(IL-12) and interleukin-18(IL-18) in gastric cancer and their association with clinicopathologic features, angiogenesis, and prognosis of gastric cancer. Methods: The expressions of IL-12 and IL-18 in 50 samples of gastric cancer tissues were analyzed by immunohistochemistry. The microvessel density(MVD) was determined with microscopic imaging analysis system. Results: The positive rates of IL-12 and IL-18 expression were 44%(22/50) and 26%(13/50), respectively. IL-12 expression was significantly correlated with pathologic differentiation(P=0.029), depth of invasion(P=0.002), lymph node metastasis(P=0.002), distant metastasis(P=0.003), and TNM stages(P=0.005). IL-18 expression was closely related to distant metastasis(P=0.001). The multivariate survival analysis revealed that IL-12, MVD and T stage were independent prognostic factors of gastric cancer. Conclusion: The positive expressions of IL-12 and IL-18 correlated with development and metastasis of gastric cancer. IL-12 is an independent factor of poor prognosis of gastric cancer.