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人肺腺癌细胞株A549和A549/DDP的比较蛋白质组学研究
         
Comparative proteomic analysis of human lung adenocarcinoma A549 and A549/DDP cells

摘    要
目的:比较人肺癌细胞株A549和顺铂(cisplatin,DDP)耐药细胞株A549/DDP中的蛋白表达差异。方法:DDP诱导A549细胞建立DDP耐药细胞株A549/DDP。应用蛋白质组学技术分离鉴定A549和A549/DDP细胞中差异表达的蛋白质,通过real-timePCR、蛋白质印迹法和免疫细胞化学法对部分差异蛋白进行验证;应用生物学信息检索分析差异蛋白的功能。结果:A549和A549/DDP细胞中有8个蛋白质点的表达差异>5倍,分别为POTE、FH(fumarate hydratase)、PDE(phosphodiesterase)、AKR1C1(aldo-keto reductase family1,memberC1)、DDH2(dihydrodiol dehydrogenase 2)、S100A10、prefoldin subunit2和核内转运蛋白,这些蛋白与细胞代谢、凋亡、增殖、解毒和信号转导等有关。Real-timePCR、蛋白质印迹法和免疫细胞化学法验证结果与蛋白质组学研究结果一致。结论:鉴定得到的A549和A549/DDP细胞中的差异蛋白为研究肺癌细胞DDP耐药提供新依据。
标    签   非小细胞肺   蛋白质组学   药物耐受性   顺铂   Carcinoma   non-small cell lung   Proteomics   Drug tolerance   Cisplatin  
 
Abstract
Objective: To compare the differentially expressed proteins between A549 cells and DDP(cisplatin)-resistant A549/DDP cells. Methods: A549 cells were exposed to DDP for developing the DDPresistantA549/DDP cells. The differentially expressed proteins in the A549 and A549/DDP cells wereseparated and identified by proteomics approach. A part of the differentially expressed proteins werevalidated by real-time PCR, Western blotting and immunocytochemistry. The functions of these proteinswere analyzed by bioinformatics method. Results: There were 8 differentially expressed proteins includingPOTE, FH(fumarate hydratase), PDE(phosphodiesterase), AKR1C1(aldo-keto reductase family 1 memberC1), DDH2(dihydrodiol dehydrogenase 2), S100A10, prefoldin subunit 2, and karyopherin beta2-Ran GppNHp nuclear transport complex between A549 and A549/DDP cells, and the difference inexpression was more than 5-fold. These proteins were related with cellular metabolism, apoptosis, cellproliferation, detoxification and signal transduction. The validation results of real-time PCR, Westernblotting and immunocytochemistry were consistent with the results of proteomic analysis. Conclusion: The differentially expressed proteins between A549 and A549/DDP cells provide some new clues for thestudy of DDP-resistant lung cancer cells.

中图分类号 R734.2   DOI 10.3781/j.issn.1000-7431.2012.03.004

 
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所属栏目 基础研究

基金项目 国家自然科学基金资助项目(编号:30873023)

收稿日期 2011/10/11

修改稿日期 2011/12/22

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引用该论文: KUANG Peng,LI Xue-fei,LI Bing,WANG Yong-sheng,LI Jia-yu,ZHOU Cai-cun. Comparative proteomic analysis of human lung adenocarcinoma A549 and A549/DDP cells[J]. Tumor, 2012, 32(3): 170~176
况鹏,李雪飞,李冰,王永生,李嘉瑜,周彩存. 人肺腺癌细胞株A549和A549/DDP的比较蛋白质组学研究[J]. 肿瘤, 2012, 32(3): 170~176


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