目的 研究DHA复合物的抗癌机理。方法 用流式细胞术研究了DHA复合物对荷瘤鼠H₂₂细胞和T淋巴细胞细胞周期及凋亡的影响。结果 (1)与阴性对照组相比,DHA复合物中、高剂量组G₀-G₁期H₂₂癌细胞百分比明显增加(P<0.01);DHA复合物低、中、高剂量组G₀-G₁期T细胞百分比明显减小(P<0.01)。(2)与阴性对照组相比,DHA复合物低、中、高剂量组S期H₂₂癌细胞百分比明显减小(P<0.01),S期T细胞百分比明显增加(P<0.01)。(3)与阴性对照组相比,DHA复合物中剂量组G₂-M期H₂₂癌细胞百分比显著减少(P<0.01),DHA复合物低、高剂量组G₂-M期H₂₂癌细胞以及DHA复合物低、中、高剂量组G₂-M期T细胞百分比均显著升高(P<0.01)。(4)与阴性对照组相比,DHA复合物低、中、高剂量组H₂₂癌细胞增殖指数(PI)明显下降(P<0.01),T细胞增殖指数明显升高(P<0.01)。(5)与阴性对照组相比,DHA复合物低、中、高剂量组H₂₂癌细胞及DHA复合物中、高剂量组T细胞凋亡率明显升高(P<0.01)。结论 DHA复合物可抑制H₂₂癌细胞的增殖,促进T淋巴细胞增殖,同时DHA复合物还可促进H₂₂细胞、T淋巴细胞凋亡

Objective To research the anti tumor mechanism of DHA compound. Methods Flow cytometry was used to study the effect of DHA compound on cell cycle and apoptosis of H₂₂ cell and T cell in mice bearing tumor. Results (1) Compared with control, the medium and high dose DHA compounds increased the percentage of G₀-G₁ phase H₂₂ cell significantly(P<0.01); the low, medium and high dose DHA compounds reduced the percentage of G₀-G₁ phase of T cell obviously(P<0.01). (2) Compared with control, the percentages of S phase of H₂₂ cell in three groups with DHA compound were lower significantly(P<0.01). But the percentages of S phase of T cell increased obviously(P<0.01). (3) Compared with control, the percentage of G₂-M phase of H₂₂ cell in DHA medium dose group was lower significantly(P<0.01).But the percentages of G₂-M phase of H₂₂ cell in the other two DHA groups and G₂-M phase of T cell in all DHA compound groups were higher obviously(P<0.01).(4) Compared with control, in DHA groups, the PI of H₂₂ cell decreased and that of T cell increased obviously(P<0.01).(5) Compared with control, the percentage of apoptosis of H₂₂ cell increased obviously in all DHA groups(P<0.01),in medium and high dose DHA groups, the T cell apoptosis rates were higher (P<0.01).Conclusion DHA compound can inhibit H₂₂ cell growth and promote T cell proliferation. At the same time, it can accelerate the apoptosis of both H₂₂ cell and T cell.