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微小RNA-218在鼻咽癌中的表达及其临床意义
         
Expression of microRNA-218 in nasopharyngeal carcinoma and its clinical significance

摘    要
目的:检测微小RNA-218(microRNA-218,miR-218)在鼻咽癌组织中的表达情况,并探讨其临床意义。方法:收集鼻咽癌活检组织标本54例,同时以35例正常及慢性炎性反应鼻咽黏膜活检组织作为对照,采用实时荧光定量PCR(real-time fluorescence quantitative PCR,RFQ-PCR)法检测鼻咽癌组织及对照组织中miR-218的表达情况,分析miR-218表达水平与鼻咽癌临床病理参数之间的关系,以及与患者预后的关系。结果:与对照鼻咽黏膜组织相比,miR-218在鼻咽癌组织中的表达水平普遍下调。鼻咽癌组织中miR-218平均表达量明显低于对照鼻咽上皮组织,差异有统计学意义(P=0.000)。miR-218表达水平在低分化鼻咽癌组织明显低于高/中分化鼻咽癌(P=0.032),临床Ⅲ/Ⅳ期鼻咽癌组织明显低于临床Ⅰ/Ⅱ期鼻咽癌(P=0.007),有转移的鼻咽癌组织明显低于无淋巴结转移的鼻咽癌(P=0.019)。Kaplan-Meier生存分析发现,miR-218表达水平越高,患者生存时间越长(P<0.05)。结论:miR-218与鼻咽癌的发生、临床进展及预后密切相关,它可能成为鼻咽癌新的潜在的治疗靶点及诊断预后分子标志物。
标    签 鼻咽肿瘤   微RNAs   基因表达   预后   miR-218   Nasopharyngeal neoplasms   microRNAs   Gene expression   Prognosis   miR-218  
 
Abstract
Objective:To investigate the expression of miR-218 (microRNA-218) in NPC (nasopharyngeal carcinoma) tissues and its clinical significance.Methods:A series of 54 tissue biopsies obtained from NPC patients and 35 control tissues with no-NPC (normal nasopharyngeal mucosa or chronic inflammation of nasopharyngeal mucosa) were collected. RFQ-PCR (real-time fluorescencebased quantitative PCR) was used to detect the miR-218 expressions in these samples. The correlations of miR-218 expression with clinicopathological characteristics and prognosis of NPC patients were analyzed.Results:Compared with the control tissues, miR-218 was consistently down-regulated in NPC tissues. The average expression level of miR-218 in NPC tissues was significantly lower than that of the control tissues (P=0.000). The expression level of miR-218 in poorly differentiated NPC was significantly reduced as compared with well/moderately differentiated NPC (P=0.032). The expression level of miR-218 in clinical stages Ⅲ/Ⅳ NPC was lower than that in clinical stages Ⅰ/Ⅱ NPC (P=0.007). Moreover, the expression level of miR-218 in NPC patients with lymph node metastasis was significantly lower than that in NPC patients without lymph node metastasis (P=0.019). NPC patients with a higher level of miR-218 expression had also a longer survival (P <0.05).Conclusion:The expression of miR-218 is associated with carcinogenesis, progression and prognosis of nasopharyngeal carcinoma, so it may be used as a potential therapeutic target, a diagnostic biomarker and a prognostic predictor in patients with NPC in future.

中图分类号 R739.62 R739.63   DOI 10.3781/j.issn.1000-7431.2013.02.012

 
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所属栏目 临床研究

基金项目 国家自然科学基金资助项目(编号:81101526)

收稿日期 2012/10/8

修改稿日期 2012/12/24

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引用该论文: DENG Min,HE Zhi-min,LIU Ji-fang,GU Yi-xue,ZHENG Guo-pei. Expression of microRNA-218 in nasopharyngeal carcinoma and its clinical significance[J]. Tumor, 2013, 33(2): 177~180
邓敏,贺智敏,刘季芳,谷依学,郑国沛. 微小RNA-218在鼻咽癌中的表达及其临床意义[J]. 肿瘤, 2013, 33(2): 177~180


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